Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands

Figure 2

SERMs decrease the in vitro differentiation of dendritic cells. Days 6-7 of the culture of cDCs grown in the regular medium and in the absence or presence of fulvestrant or tamoxifen. (a) Representative plot of the flow cytometry analysis of CD11c and CD11b expression in the alive gate of cDCs generated in standard medium supplemented with 100 nM fulvestrant or 100 nM tamoxifen. (b–g) MdFI of CD11c (b, e); CD11b (c, f); manual counts of alive cells (d, g). Results are averages and SE of biological triplicates conducted with one independent cDC culture. Statistical significance was calculated by one-way ANOVA and post hoc multiple comparison test against the 0 condition (no E2 nor BPA) in a-0063 and against control in (d–i). represents , for , for .

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