Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands

Figure 3

Estrogen but not BPA regulates the production of proinflammatory cytokines by cDCs upon TLR stimulation. We grew cDCs in the absence or presence of low (0.05 nM) and high doses (50 nM) of E2 or BPA for 6-7 days (a–d) or in regular medium in the presence or absence of SERMs fulvestrant or tamoxifen (e-f). We then stimulated cDCs with either 100 ng/mL of LPS (a-b) or 10 μg/mL of CpG (c–f) and measured IL-12p70 and TNFα levels by ELISA in DC culture supernatants 24 hours post stimulation. Results are the averages and SE of four DC cultures (a-b, c-d) and two DC cultures with two biological duplicates each (e-f). Statistical significance was calculated by one-way ANOVA and post hoc multiple comparison test against 0 condition (no E2 nor BPA) or control. DMSO and EtOH were used as vehicle controls. represents , for , and for .

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