Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands

Figure 5

Estrogen but not BPA increases the gene expression of TLR9. We grew cDCs in the absence or presence of low (0.05 nM) and high doses (50 nM) of E2 or BPA for 6-7 days. Then we measured the total RNA expression of ERalpha1 (a), Tlr4 (b), and Tlr9 (c) by real-time quantitative RT-PCR. Results are averages and SE of two cDC cultures. (d) and (e) show the results of the real-time quantitative RT-PCR for TLR4 and TLR9 transcripts in cDCs grown in the presence of tamoxifen or fulvestrant. Results are averages and SE of three cDC cultures. We calculated statistical significance by one-way ANOVA and post hoc multiple comparison test against 0 condition (no E2 nor BPA) in (a–c) and against control in (d-e). Since the three controls medium alone, ethanol, and DMSO gave comparable results, we pooled the data. represents , for , and for .

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