Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells

Figure 3

Representative results depicting the effects of TMA analogues (1–26) at 12.5–100 μM concentrations, compared to TMA in EMSA experiments on the molecular interactions between NF-κB p50 and ³²P-labeled target NF-κB double-stranded oligonucleotide. Compounds 2, 3, 6, 7, 11, 14, 15, 17–22, and 24 were not analyzed because the relative EMSA experiments demonstrated low activity (MIC > 100 μM). Arrow indicates NF-κB/DNA complexes; asterisk indicates the free ³²P-labeled target NF-κB probe.