Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells

Table 3

Results of RT-qPCR analysis reporting the % inhibition of IL-8 mRNA in TNF-α-induced IB3-1 cells cultured with 1 μM and 10 μM concentrations of TMA derivatives.

Derivative	1 μM (% inhibition)	10 μM (% inhibition)	Inhibition of NF-κB/DNA complex (MIC) μM
1	57	56	<12.5 ()
5	33	31	12.5–25
8	51	52	25–50
12	0	na	12.5
16	18	21	25–50
23	3	11	2–505
25	na	na	50–100
26	55	83	50–100
27	16	na	<12.5 ()
28	21	36	12.5–25
29	na	12	<12.5 ()
30	10	na	12.5–25
31	na	na	12.5
32	73	52	<12.5 ()
33	68	67	12.5–25
34	66	47	<12.5 ()
35	56	37	25–50
36	77	74	12.5–25
37	na	na	12.5–25
38	36	81	25

na: not active at the used concentrations; MIC: minimal inhibitory concentration. In the EMSA experiments, 12.5 μM was the minor concentration used.