Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells

Figure 7

PNU-282987 downregulated nicotine-induced expression of matrix metalloproteinase- (MMP-) 9, MMP-2, monocyte chemotactic protein- (MCP-) 1, and regulated upon activation normal T cell expressed and secreted (RANTES) in MOVAS cells. (a) MOVAS cells were pretreated with 10 μm PNU-282987 for 60 min prior to 10 ng/ml nicotine exposure for 3 h. The band optical density values (means ± SD) of (b) MMP-9, (c) MMP-2, (d) MCP-1, and (e) RANTES in the Western blot were quantified by using ImageJ software and normalized to GAPDH. Representative results from three independent experiments are shown. (f) MMP-9, (g) MMP-2, (h) MCP-1, and (i) RANTES mRNA levels were determined by real-time reverse transcription polymerase chain reaction. All experiments were analyzed as triplicate independent experiments. and versus controls; ^# and ^## versus the group treated with nicotine.

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