MCL Plays an Anti-Inflammatory Role in Mycobacterium tuberculosis-Induced Immune Response by Inhibiting NF-κB and NLRP3 Inflammasome Activation

Figure 2

MCL suppresses the expression of iNOS and COX2 induced by Mtb infection both at transcriptional and at translational levels. Raw264.7 cells (1 × 10⁶/well) were seeded in 6-well plates overnight. Four hours after infected with H37Ra at MOI of 10, cells were washed with PBS and treated with different concentration of MCL (5, 10, and 20 μM) for 12 hr. The mRNA expression of iNOS (a) and COX2 (b) was detected by qRT-PCR. Data are shown as the means ± SD (). .The protein levels of iNOS (c) and COX2 (d) were detected by Western blot, and β-actin was used as an internal control. (e) and (f) showed the statistical results for iNOS and COX2 expression. Data were presented as mean ± SD in at least three independent experiments. .

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