Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis

Figure 2

The mRNA and protein level of NLRP3, caspase-1, procaspase-1, IL-1β, and pro-IL-1β in each group. (a) RT-PCR detected the expression of NLRP3, caspase-1, and IL-1β mRNA after intervened by high glucose and maresin 1 for 24 h in GMCs, and the mRNA expression of NLRP3, caspase-1, and IL-1β increased in HG group significantly, but compared with HG group, it decreased obviously in M + HG group as a concentration-dependent manner of maresin 1. (b) The corresponding relative gray value statistics graph of the mRNA level. versus NC group, versus HG group. (c) Western-blot detected the expression NLRP3, caspase-1, procaspase-1, IL-1β, and pro-IL-1β protein after intervened by high glucose and maresin 1 for 24 h in GMCs, and the protein expression of NLRP3, caspase-1, and IL-1β increased and procaspase-1 and pro-IL-1β decreased in HG group significantly, but compared with HG group, the protein expression of NLRP3, caspase-1, and IL-1β decreased and procaspase-1 and pro-IL-1β increased obviously in M + HG group as a concentration-dependent manner of maresin 1. (d) The corresponding relative gray value statistics graph of the protein level. versus NC group, versus HG group.

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