Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis

Figure 4

The level of NLRP3, caspase-1, P-caspase-1, IL-1β, and P-IL-1β in each group. (a) RT-PCR detected the expression NLRP3, caspase-1, and IL-1β mRNA after intervened by high glucose and 100 nmol/L maresin 1 in GMCs, and in HG group, the mRNA expression of NLRP3, caspase-1, and IL-1β was higher than that in NC group, but for M3 + HG group, the expression reduced visibly compared to the HG group. (b) The corresponding relative gray value statistics graph of the mRNA level. versus NC group, versus HG group. (c) Western-blot detected the expression of NLRP3, caspase-1, P-caspase-1, IL-1β, and P-IL-1β protein after intervened by high glucose and 100 nmol/L maresin 1 in GMCs, and the protein expression of NLRP3, caspase-1, and IL-1β was higher than that in NC group, but for M3 + HG group, the expression reduced visibly compared to the HG group. The expression of P-caspase-1 and P-IL-1β was just the reverse. (d) The corresponding relative gray value statistics graph of the protein level. versus NC group, versus HG group.

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