Neutral Sphingomyelinase Behaviour in Hippocampus Neuroinflammation of MPTP-Induced Mouse Model of Parkinson’s Disease and in Embryonic Hippocampal Cells

Figure 3

Sphingomyelin in control and vitamin D3-treated HN9.10 cells after 48 h of culture. (a) SM species studied by using 16:0SM, 18:1SM, and 24:0SM external calibrators. Data are expressed as nmol/mg protein and represent the mean ± S.D. of three separated experiments. (b) SM species studied by evaluating the areas of all the peaks identified on the basis of their molecular weight. Data are expressed as area/mg protein and represent the mean ± S.D. of three separated experiments. (c) Total saturated and unsaturated fatty acids. Data are expressed as area/mg protein and represent the mean ± S.D. of three separated experiments. versus control sample. (1) 14:0SM; (2) 16:0SM; (3) 16:1SM; (4) 16:3SM; (5) 16:4SM; (6) 18:0SM; (7) 18:1SM; (8) 18:4SM; (9) 20:0SM; (10) 20:4SM; (11) 20:5SM; (12) 22:0SM; (13) 22:1SM; (14) 22:2SM; (15) 22:6SM; (16) 24:0SM; (17) 24:1SM; (18) 24:2SM; (19) 24:3SM; (20) 24:4SM; (21) 26:0SM; (22) 26:2SM; (23) 26:3SM; (24) 26:4SM.

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