Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients

Figure 5

Whole blood from healthy volunteers (HV, ) or major trauma patients (TP, ) was analyzed by flow cytometry over a 10-day time course after admission (emergency department, ED–10). Monocytes were detected using anti-human CD14 in the corresponding sideward and forward scatter. Unstimulated (black symbols) and stimulated (clear symbols) measurements were made. For stimulation (stim), whole blood was incubated with leukocyte activation cocktail for 5 h with subsequent analyzing procedure as in unstimulated samples. Data are shown as mean ± SEM. (a) TLR2/HLA-DR coexpression on CD14⁺ monocytes, (b) TLR4/HLA-DR coexpression on CD14⁺ monocytes, and (c) TLR9/HLA-DR coexpression on CD14⁺ monocytes. versus unstimulated HV; TP versus corresponding TP stim; versus all; TP stim versus corresponding unstimulated TP and HV stim.

(a)

(b)

(c)