Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium

Table 1

Oligonucleotide primers and amplicon length of PCR products.

Gene information	Primer sequence	Amplicon length	Accession number	Reference
TNF-α (tumor necrosis factor α,; Sus scrofa)	(S) 5′-GCT GTA CCT CAT CTA CTC CC-3′	291 bp	NM_214022.1	[22]
	(AS) 5′-TAG ACC TGC CCA GAT TCA GC-3′
IL-6 (interleukin 6, Sus scrofa)	(S) 5′-ATG CTT CCA ATC TGG GTT CA-3′	198 bp	M80258.1
	(AS) 5′-GTG GTG GCT TTG TCT GGA TT-3′
IL-1α (interleukin 1α, Sus scrofa)	(S) 5′-CAA GGA CAG TGT GGT GAT GG -3′	167 bp	NM_214029.1
	(AS) 5′-TCA TGT TGC TCT GGA AGC TG-3′