Mediators of Inflammation / 2017 / Article / Tab 2 / Research Article
Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium Table 2 Primary and secondary antibodies (WB = Western blot, IF = immunofluorescence).
Antigen Species Company (catalog #) Dilution WB Dilution IF Primary antibodies Claudin-1 Rabbit Invitrogen/Thermo Fisher Scientific (51-9000) 1 : 1000 Claudin-3 Rabbit Invitrogen/Thermo Fisher Scientific (34-1700) 1 : 1000 1 : 200 Claudin-4 Mouse Invitrogen/Thermo Fisher Scientific (32-9400) 1 : 5000 1 : 200 Claudin-5 Mouse Invitrogen/Thermo Fisher Scientific (35-2500) 1 : 1000 Claudin-7 Rabbit Invitrogen/Thermo Fisher Scientific (34-9100) 1 : 2000 Claudin-8 Rabbit Invitrogen/Thermo Fisher Scientific (40-2600) 1 : 1000 Occludin Rabbit Invitrogen/Thermo Fisher Scientific (71-1500) 1 : 200 β -actinMouse Sigma (A5441) 1 : 10,000 Antigen Species Company (catalog #) Conjugate Dilution WB Dilution IF Secondary antibodies Mouse IgG Goat Jackson Immuno Research (115-225-146) Cy2 1 : 600 Rabbit IgG Goat Jackson Immuno Research (111-175-144) Cy5 1 : 600 Mouse IgG Goat Jackson Immuno Research (115-035-003) HRP 1 : 10,000 Rabbit IgG Goat Jackson Immuno Research (111-036-003) HRP 1 : 10,000
