Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions

Figure 5

Impact of CM on uptake/replication of H37Rv within PMN. PMN () cultured for 1.5 h alone (N-T) or with control-CM, H37Rv-CM, or M-CM were infected or not with H37Rv at MOI of 3 for 1 h. Then noninfected or H37Rv-infected PMN were collected (0 h) or cultured for further 18 h. Numbers of CFU recovered from 0 h (uptake) or 18 h upon infection were determined as described in Materials and Methods. Median ± SEM is shown. Statistical differences: for PMN preincubated with M-CM versus NT-PMN.