Figure 4: Epac1 and NLRP3 both can reduce cleavage of caspase-1 and IL-1β. Protein analyses from REC grown in normal glucose (NG), high glucose (HG), high glucose + Epac1 agonist (HG + 8-cpt-cAMP), high glucose + NLPR3 siRNA (HG + NLRP3 siRNA), high glucose + scrambled siRNA (HG + Sc), and high glucose + Epac1 agonist + NLRP3 siRNA (HG + NLRP3 siRNA + 8-cpt-cAMP) for NLRP3 (a), cleaved caspase-1 (b), and IL-1β (c). for NLRP3 and cleaved caspase-1; for ELISA for IL-1β. Data are mean ± SEM. versus NG; versus HG; versus HG+8-cpt-cAMP.