Stigmasterol Modulates Allergic Airway Inflammation in Guinea Pig Model of Ovalbumin-Induced Asthma

Figure 2

Effect of stigmasterol on serum vascular cell adhesion molecule-1 (VCAM-1) and serum OVA-specific immunoglobulin E (OVA sIgE). Guinea pigs were sensitized and challenged with ovalbumin as described in the methods. Animals received either saline, dexamethasone, or stigmasterol 1 h prior to each challenge. Naïve controls received normal saline only. 24 h after the last challenge, animals were bled by dissection of the jugular vein. Blood collected was allowed to clot and centrifuged at 1000 rpm for 15 min. Serum vascular cell adhesion molecule-1, VCAM-1 (a), and serum OVA-specific immunoglobulin E, OVA sIgE (b), levels were quantified with sandwich ELISA. Data is expressed as VCAM-1 or OVA sIgE concentration (pg/ml) ± SEM (). , , and as compared to saline-treated control; ^### as compared to naïve control using one-way ANOVA followed by Dunnet’s post hoc test.

(a)

(b)