Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells

Figure 6

Quantitative real-time PCR (qPCR) analysis of the expression of IL-18, IL-18BP, and IL-18 receptor (IL-18R) mRNA in HMC-1 mast cells. Cells were challenged by various concentrations of group 1 house dust mite allergen Der P 1, ovalbumin (OVA), or calcium ionophore (CI) at 37°C for 2 h and 16 h. The expression of IL-18 (a), IL-18BP (b), and IL-18R (c) mRNAs were analyzed by qPCR. The data were expressed as mean ± SE for four separate experiments performed in duplicate. compared with the response to corresponding medium alone control. in comparison of the reduced response with the response to the corresponding allergen alone. in comparison of the enhanced response with the response to the corresponding allergen alone.

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