Molecular Responses of Human Retinal Cells to Infection with Dengue Virus

Figure 4

Antiviral type I interferon (IFN) response of human retinal pigment epithelial cells and human retinal endothelial cells infected with DENV: viral strain = Mon601; multiplicity of infection = 1; evaluated time points postinoculation = 24, 48, and 72 hours (hr). (a) and (b) Graphs showing IFN-β protein concentration in culture supernatant of (a) DENV-infected retinal pigment epithelial cells and (b) DENV-infected endothelial cells. Bars represent mean protein concentration, with error bars showing standard deviation. cultures/condition. No IFN-β protein was detected in mock-infected cells. ND = not detectable. (c) and (d) Graphs showing relative transcript expression for IFN-stimulated gene products in (c) DENV-infected versus mock-infected retinal pigment epithelial cells and (d) DENV-infected versus mock-infected endothelial cells. Reference genes were glyceraldehyde-3-phosphate dehydrogenase and TATA-binding protein. Bars represent mean relative expression, with error bars showing standard deviation. cultures/condition. Data were analyzed by two-tailed Student’s -test. IFITM1 = IFN-induced transmembrane protein 1; EIF2AK2 = eukaryotic translation initiation factor 2-alpha kinase 2; RSAD2 = radical SAM domain-containing 2 (also known as viperin); ISG15 = IFN-stimulated gene 15.