Molecular Responses of Human Retinal Cells to Infection with Dengue Virus
Cell adhesion molecule expression by human retinal endothelial cells infected with DENV: viral strain = Mon601; multiplicity of infection = 1; evaluated time points postinoculation = 24, 48, and 72 hours (hr). (a) Graphs showing relative transcript expression for adhesion molecules in DENV-infected versus mock-infected endothelial cells. Reference genes were glyceraldehyde-3-phosphate dehydrogenase and TATA-binding protein. Bars represent mean relative expression, with error bars showing standard deviation. cultures/condition. Data were analyzed by two-tailed Student’s -test. (b) Graph showing relative expression of VCAM-1 protein on the surface of DENV-infected versus mock-infected endothelial cells. Each pair of circles joined by a line represents the mean expression for DENV-infected versus mock-infected endothelial cells for one of 3 independent experiments. cultures/condition. Data were analyzed by one-tailed paired Student’s -test. ICAM-1 = intercellular adhesion molecule, 1; VCAM-1 = vascular cell adhesion molecule 1; ALCAM = activated leukocyte cell adhesion molecule.
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