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Mediators of Inflammation
Volume 2017 (2017), Article ID 3164375, 16 pages
https://doi.org/10.1155/2017/3164375
Research Article

Molecular Responses of Human Retinal Cells to Infection with Dengue Virus

1Microbiology & Infectious Diseases, Flinders University School of Medicine, Rm 5D-316, 1 Flinders Drive, Bedford Park, Adelaide, SA 5042, Australia
2Eye & Vision Health, Flinders University School of Medicine, Rm 4E-431, 1 Flinders Drive, Bedford Park, Adelaide, SA 5042, Australia
3Flinders Centre for Innovation in Cancer, Flinders University School of Medicine, 1 Flinders Drive, Bedford Park, Adelaide, SA 5042, Australia
4Ocular Inflammation and Immunology Service, Singapore National Eye Centre, 11 Third Hospital Avenue, Singapore 168751

Correspondence should be addressed to Justine R. Smith

Received 27 April 2017; Revised 22 August 2017; Accepted 6 September 2017; Published 12 November 2017

Academic Editor: Giuseppe Valacchi

Copyright © 2017 Jillian M. Carr et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. Infection of human retinal pigment epithelial cells and human retinal endothelial cells with patient DENV-2 isolate: viral strain = PUO-312; multiplicity of infection = 1; evaluated time point post-inoculation = 48 hours. (A) and (B) DENV- and mock-infected (A) retinal pigment epithelial cells and (B) retinal endothelial cells immunolabeled to detect double stranded (ds)RNA and DENV antigen (Ag). Alexa Fluor 555 (red) and Alexa Fluor 488 (green) with Hoechst 33342 nuclear counterstain (blue). Original magnification: 630x. (C) and (D) Graphs showing expression of selected transcripts in DENV-infected (C) retinal pigment epithelial cells and (D) retinal endothelial cells, versus mock-infected cells. Reference genes were glyceraldehyde-3-phosphate dehydrogenase and TATA-binding protein. Bars represent mean relative expression, with error bars showing standard deviation. n = 3 cultures/condition. Data were analyzed by two-tailed Student’s t-test. TNF-α = tumor necrosis factor-α, IFN-β = interferon-β, VCAM-1 = vascular cell adhesion molecule 1, CTNNB1 = catenin-β1.

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