Inhibition of Murine Pulmonary Microvascular Endothelial Cell Apoptosis Promotes Recovery of Barrier Function under Septic Conditions

Figure 2

Time course of cytomix-induced mouse PMVEC hyperpermeability. Cytomix-stimulated PMVEC had significantly increased permeability by 4 h after stimulation versus PBS by 3 assays: lower TEER (a) and higher macromolecular flux including fluorescein isothiocyanate- (FITC-) labelled dextran (b) and EB-labelled albumin (c). Interestingly, leak as assessed by TEER appeared to recover by 16–24 h after cytomix, whereas septic enhanced macromolecular flux persisted. represents compared with PBS (two-way ANOVA). .

(a)

(b)

(c)