Inhibition of Murine Pulmonary Microvascular Endothelial Cell Apoptosis Promotes Recovery of Barrier Function under Septic Conditions

Figure 4

Caspase activation correlates with DNA fragmentation as a marker of PMVEC apoptosis. (a) Cytomix stimulation leads to an increased number of cells stained positive for both active caspases (FLICA; red) and fragmented DNA (TUNEL; green). Note that overlap between the 2 markers of apoptosis appears as yellow-white. Nuclei were stained with Hoechst 33342. (b) Quantitation of double-positive cells revealed that at 16 h, only of FLICA+ cells were also TUNEL+, whereas by 24 h, this number had increased to . Interestingly, almost all TUNEL+ cells were FLICA+ at both 16 h () and 24 h (). .

(a)

(b)