Cyanidin-3-O-Glucoside Modulates the In Vitro Inflammatory Crosstalk between Intestinal Epithelial and Endothelial Cells

Figure 3

(a) TNF-α and (b) IL-8 gene expression in Caco-2 cells. The Caco-2 cell monolayer was pretreated for 24 hours with 20 μM C3G or with 25 μM WED for one hour and subsequently exposed to 50 ng/mL TNF-α for one hour. Cultures treated with the vehicle alone (0.1% DMSO) were used as controls (CTR). Results, deriving from three independent experiments, are expressed as (mean ± SD). 18S rRNA was used as housekeeping gene. versus CTR; versus TNF-α.

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