FTY720 Attenuates Angiotensin II-Induced Podocyte Damage via Inhibiting Inflammatory Cytokines

Figure 3

FTY720 decreased S1P synthesis in Ang II-infusion rats via downregulation of inflammatory cytokines. Subcutaneous continuous Ang II-infusion at 400 ng/kg⋅min concentration in rats for day 28: we sacrificed rats at day 28. FTY720 (0.5 g/kg⋅d) was orally administered to rats for 28 days and at the same time rats also received Ang II-infusion. When the mice were sacrificed, the kidney was fixed in paraformaldehyde; immunohistochemical analysis of TNF-α and IL-6 protein expression in kidney sections. Rat blood samples were collected and plasma was separated for analyzing S1P concentration by ELISA and SPHK1 protein expression level by western blot. Part of the rat kidney tissue homogenate was used to detect IL-6 and TNF-α mRNA expression level. Data represented is the means ± SD of 6 independent experiments performed in triplicate. () , versus normal saline infusion control rats; , versus Ang II-infusion rats. (a) S1P concentration in rat plasma were analyzed by ELISA. (b) TNF-α and IL-6 were detected by immunohistochemical in rat kidney. (c) IL-6 mRNA expression level was detected by RT-PCR in rat kidney tissue homogenate. (d) TNF-α mRNA expression level was detected by RT-PCR in rat kidney tissue homogenate. (e) SPHK1 protein expression level in rat plasma was detected by western blot. β-Actin was used to ensure an equal amount of protein was loaded in each lane. Data represented is the means ± SD of six independent experiments performed in triplicate.

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