Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages

Figure 2

Effects of HMFO on colonic histology and MPO levels in mice with DSS-induced colitis. (a) Histological changes of colonic tissue were determined by hematoxylin and eosin (H&E) staining and observation by light microscopy (×20 and ×40). Left panel: low-power view (scale bar = 100 μm). Right panel: high-power view (scale bar = 50 μm). (b) Histological scores were assigned according to the criteria defined in Section 2.5. (c) MPO was extracted from colon tissue specimens and assayed for colorimetric activity, with the activity determined as the units per gram of colonic tissue. Values with different letters are significantly different (). 5-ASA, 5-aminosalicylic acid (100 mg/kg); DSS, dextran sulfate sodium; HMFO, polysaccharides of Cynanchum wilfordii (100 or 200 mg/kg); MPO, myeloperoxidase.

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