Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages

Figure 7

Effects of HMFO on the NF-κB signaling pathways in LPS-induced RAW 264.7 macrophages. RAW 264.7 macrophages were pretreated with different concentrations of HMFO for 2 h and then stimulated with LPS for 22 h. Phosphorylation of (a) p65, (b) IκB-α, and (c) IKK α/β was analyzed by Western blotting. The NF-κB DNA binding activies (b) were measured by NF-κB (p65) transcription factor assay kit as described in Materials and Methods. Values with different letters are significantly different ().

(a)

(b)

(c)

(d)