15-Deoxy-Δ^12,14-prostaglandin J₂ Exerts Antioxidant Effects While Exacerbating Inflammation in Mice Subjected to Ureteral Obstruction

Figure 2

15d-PGJ₂ enhances the proinflammatory response during 3-day UUO (3dUUO). (a) Nuclear protein extracts were used to analyze cortical NF-κB P65 subunit nuclear protein expression (Nuc). (b) Cortical cytoplasmic protein expression (Cyt) of the NF-κB P65 subunit. (c) Cortical cytoplasmic protein expression of IκBα. (d) F4/80 mRNA levels in the kidney cortex. (e) Ly6G mRNA levels in the cortex. (f) Mcp-1 mRNA levels in the cortex. (g) Tnf-α mRNA levels in the cortex. Representative blots are shown above each graph. 15d-PGJ₂ was administered at a low (0.5 mg/kg/day) and a high (1 mg/kg/day) dose as depicted in each graph. Data represents mean ± SEM. versus that in sham; versus that in UUO, using ANOVA and Bonferroni’s post hoc tests (, sham; , UUO groups). UUO, unilateral ureteral obstruction.

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