The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression
The impact of parenteral Vit C on CD4+ T cells-mediated cellular immunosuppression in sepsis. Treatment with 200 mg/Kg parenteral Vit C upregulated the proliferative response of CD4+CD25− T cells of WT mice and Gulo−/− mice in sepsis (a). The apoptotic rate of splenic CD4+CD25− T cells was analyzed with Annexin-V-FITC/PI by flow cytometry at 24 hours after CLP-induced midgrade sepsis (e). The apoptotic level of splenic CD4+CD25− T cells was decreased when 200 mg/Kg parenteral Vit C was administered after CLP to WT mice and Gulo−/− mice in sepsis (b). Parenteral Vit C increased the serum level of IFN-γ (c) as well as decreasing the serum levels of IL-4 (d) of WT mice and Gulo−/− mice in sepsis. Data were represented as mean ± standard deviation (SD) and analyzed by software SPSS 17.0 with one-way ANOVA, per group, or .