Mediators of Inflammation / 2017 / Article / Tab 1

Research Article

The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

Table 1

Targeted genes and their primer sequences for SYBR Green and Methylation-Sensitive Real-Time Polymerase Chain Reaction.

GenePrimer sequences

Mouse Foxp-3Forward: 5′-CAGCTGCCTACAGTGCCCCTAG-3
Reverse: 5′-CATTTGCCAGCAGTGGGTAG-3′
Mouse HeliosForward: 5′-TAAGCTCAGCTTATTCTCAGGTCTATCA-3′ 
Reverse: 5′-ATGTTGTTTTCGTGACTATCAGATGTT-3′
Mouse CTLA-4Forward: 5′-CGCAGATTTATGTCATTGATCC-3′ 
Reverse: 5′-TTTTCACATAGACCCCTGTTGT-3′
Mouse TGF-βForward: 5′-AACAATTCCTGGCGTTACCTT-3′ 
Reverse: 5′-GAATCGAAAGCCCTGTATTCC-3′
Mouse IL-10Forward: 5′-ACAGCCGGGAAGACAATAAC-3′ 
Reverse: 5′-CAGCTGGTCCTTTGTTTGAAAG-3′
Mouse Foxp3-TSDRForward: 5′-CTTGCTTCCTGGCACGAGATTTGAATTGGATATGGTTTGT-3′ 
Reverse: 5′-CAGGAAACAGCTATGACAACCTTAAACCCCTCTAACATC-3