Distinct Signature of Oxylipid Mediators of Inflammation during Infection and Asymptomatic Colonization by E. coli in the Urinary Bladder

Figure 2

Uropathogenic E. coli infection and asymptomatic bacteriuric E. coli colonization in mice used for lipidomic analyses. (a, b) Female mice () were inoculated with UPEC (CFT, closed circles) or ABEC (ABU, open circles), and urinary PMN cell count was determined at 6 h (early acute) and 48 h (late acute) postinoculation by Turk’s staining. PMNs were counted with a hemacytometer. Each symbol represents PMN count in a mouse, and bars correspond to median. Dotted line denotes the limit of detection (2500 PMN/ml). , Kruskal-Wallis test with Dunn’s posttest. (c) Weight of urinary bladders (urine-free) from UPEC-infected and ABEC-colonized mice was determined at 6 h and 48 h PI. These bladders were used for lipidomic analyses. Bars represent mean, and error bars correspond to SEM. , ANOVA with Dunnett’s posttest. PBS, phosphate-buffered saline-inoculated control; CFT, uropathogenic E. coli strain CFT073; ABU, asymptomatic bacteriuria E. coli strain 83972; PMN, polymorphonuclear cell; and h PI, hour postinoculation.

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