Distinct Signature of Oxylipid Mediators of Inflammation during Infection and Asymptomatic Colonization by E. coli in the Urinary Bladder

Figure 6

Oxylipid profile of urinary bladders during colonization and infection by E. coli. Heatmap depicting fold change in the concentration of 39 oxylipid metabolites in mouse urinary bladders during asymptomatic bacteriuria (ABU) and infection (CFT) by E. coli, compared to PBS-inoculated controls, was generated using HeatmapGenerator5. Fold change in abundance compared to PBS controls was calculated and log transformed to generate this heatmap. Substrate and pathways involved in the generation of oxylipid metabolites are also indicated. Adult female CBA/J mice () were used for the early (6 h postinoculation) and late (48 h postinoculation) time points. ABU, asymptomatic bacteriuric E. coli strain 83972; CFT, uropathogenic E. coli strain CFT073; ArA, arachidonic acid; LnA, linoleic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; ALA, α-linolenic acid; COX, cyclooxygenase; CYP, cytochrome P450 epoxygenase; sEH, soluble epoxide hydrolase; LOX, lipoxygenase; and NE, nonenzymatic reaction. For analytes that were below the limit of detection (6 h: 9-HETE and 11-HETE; and 48 h: 12-HETE, 15-HETrE, 13-HOTrE, and 15-HEPE), fold change was set at 0. Chemical names of oxylipids are listed in the Materials and Methods.