Distinct Signature of Oxylipid Mediators of Inflammation during Infection and Asymptomatic Colonization by E. coli in the Urinary Bladder
Table 1
Oxylipidome of the urinary bladder during E. coli colonization and infection.
Substrate: pathway
Early, 6 h PI
Late, 48 h PI
Oxylipid
PBS
ABU
UTI-CFT
PBS
ABU
UTI-CFT
ArA:COX
PGE2
1.94 ± 0.2
1.99 ± 0.5
8.55 ± 2.4
1.03 ± 0.2
5.02 ± 1.6
3.99 ± 1.3
ArA:COX
PGD2
1.24 ± 0.08
1.31 ± 0.3
5.64 ± 1.6
0.93 ± 0.2
5.3 ± 1.7
4.27 ± 1.4
ArA:COX
12-HHTrE
0.42 ± 0.1
0.35 ± 0.1
2.53 ± 0.58
0.16 ± 0.03
3.90 ± 1.1
3.08 ± 1.2
ArA:COX
TXB2
0.11 ± 0.01
0.047 ± 0.03
0.29 ± 0.06
0.024 ± 0.01
0.21 ± 0.06
0.28 ± 0.01
ArA:COX
6-ketoPGF1α
7.66 ± 2.07
4.4 ± 1.4
42.5 ± 10.6
1.3 ± 0.3
5.55 ± 1.17
5.32 ± 1.6
ArA:CYP
8,9-EET
0.81 ± 0.1
0.46 ± 0.1
2.08 ± 0.53
0.24 ± 0.06
0.30 ± 0.10
0.62 ± 0.1
ArA:CYP-sEH
8,9-DHET
0.24 ± 0.1
0.056 ± 0.04
1.24 ± 0.52
0.10 ± 0.03
0.24 ± 0.1
0.27 ± 0.08
ArA:CYP
11,12-EET
0.60 ± 0.1
0.33 ± 0.01
1.15 ± 0.14
0.10 ± 0.03
0.04 ± 0.01
0.22 ± 0.09
ArA:CYP-sEH
11,12-DHET
0.88 ± 0.16
0.4 ± 0.2
3.3 ± 0.67
0.001 ± 0.003
0.12 ± 0.1
0.002 ± 0.003
ArA:CYP
14,15-EET
0.73 ± 0.1
0.38 ± 0.14
1.73 ± 0.42
0.2 ± 0.05
0.065 ± 0.01
0.32 ± 0.13
ArA:CYP-sEH
14,15-DHET
2.03 ± 0.66
0.79 ± 0.2
5.04 ± 0.89
0.29 ± 0.001
0.66 ± 0.13
0.56 ± 0.07
ArA:CYP
20-HETE
0.047 ± 0.01
0.054 ± 0.01
0.114 ± 0.02
0.30 ± 0.13
0.51 ± 0.15
0.13 ± 0.03
LnA:CYP
9,10-EpOME
0.199 ± 0.1
0.1 ± 0.02
0.349 ± 0.08
0.03 ± 0.001
0.03 ± 0.01
0.06 ± 0.01
LnA:CYP-sEH
9,10-DiHOME
1.43 ± 0.29
0.54 ± 0.19
2.3 ± 0.24
0.09 ± 0.01
0.45 ± 0.14
0.32 ± 0.01
LnA:CYP
12,13-EpOME
0.188 ± 0.03
0.08 ± 0.02
0.368 ± 0.10
0.02 ± 0.005
0.03 ± 0.009
0.05 ± 0.01
LnA:CYP-sEH
12,13-DiHOME
2.98 ± 0.78
0.55 ± 0.16
2.95 ± 0.88
0.22 ± 0.09
0.98 ± 0.45
0.76 ± 0.21
DHA:CYP
19,20-EPDPE
0.96 ± 0.29
0.67 ± 0.13
1.63 ± 0.68
0.84 ± 0.01
1.08 ± 0.040
1.12 ± 0.09
DHA:CYP
19,20-DiHDPA
8.88 ± 2.1
2.44 ± 0.44
16.51 ± 6.53
0.92 ± 0.2
2.60 ± 0.53
3.11 ± 0.32
EPA:CYP
5,6-DiHETE
0.27 ± 0.22
0.25 ± 0.21
0.42 ± 0.1
0.023 ± 0.1
0.19 ± 0.07
0.11 ± 0.04
EPA:CYP
14,15-DiHETE
1.97 ± 1.1
0.57 ± 0.14
2.84 ± 1.4
0.31 ± 0.01
1.15 ± 0.32
0.69 ± 0.20
EPA:CYP
17,18-DiHETE
16.48 ± 5.16
4.18 ± 1.89
25.22 ± 3.98
2.52 ± 0.43
6.19 ± 1.18
5.04 ± 0.79
ArA:LOX
5-HETE
10.9 ± 1.39
6.3 ± 1.6
22.15 ± 2.6
3.68 ± 0.78
10.89 ± 2.56
7.16 ± 1.04
ArA:LOX
5-oxoETE
2.3 ± 0.24
1.59 ± 0.27
5.99 ± 0.44
0.56 ± 0.02
1.49 ± 0.34
1.5 ± 0.17
ArA:LOX
12-HETE
290.6 ± 82.1
192.4 ± 80.1
503.5 ± 95.8
BLD
BLD
BLD
ArA:LOX
15-HETE
9.65 ± 2.1
7.42 ± 1.37
9.04 ± 1.91
9.36 ± 2.29
23.59 ± 5.83
20.86 ± 3.9
ArA:LOX
15-oxoETE
6.72 ± 0.7
3.88 ± 1.0
13.99 ± 2.2
1.85 ± 0.66
2.8 ± 0.9
4.32 ± 1.04
ArA:LOX
5,6-LipoxinA4
0.397 ± 0.1
0.139 ± 0.05
0.75 ± 0.37
0.162 ± 0.06
0.753 ± 0.23
0.403 ± 0.09
LnA:LOX
9-HODE
34.83 ± 8.84
18.48 ± 11.1
38.49 ± 12.0
17.62 ± 6.94
34.1 ± 11.71
26.72 ± 5.54
LnA:LOX
9-oxoODE
113.4 ± 26.2
41.8 ± 14.25
198.3 ± 61.9
17.41 ± 3.48
33.2 ± 8.8
52.9 ± 7.25
LnA:LOX
13-HODE
42.62 ± 10.5
22.41 ± 12.41
41.75 ± 12.1
28.9 ± 11.4
47.7 ± 16.8
43.76 ± 9.5
LnA:LOX
13-oxoODE
38.33 ± 8.63
15.1 ± 4.7
61.87 ± 19.6
5.2 ± 1.2
7.77 ± 1.95
12.7 ± 1.5
DHA:LOX
RvD2
0.053 ± 0.03
0.087 ± 0.03
0.077 ± 0.02
0.044 ± 0.02
0.068 ± 0.02
0.05 ± 0.01
DHA:LOX
17-HDoHE
6.29 ± 2.1
5.45 ± 0.53
3.11 ± 0.72
2.91 ± 1.08
7.75 ± 1.69
9.49 ± 2.93
DHA:LOX
10,17-DiHDoHE
34.82 ± 20.8
41.17 ± 22.9
41.64 ± 15
0.099 ± 0.04
0.05 ± 0.01
0.19 ± 0.07
ALA:LOX
15-HETrE
24.1 ± 7.26
9.93 ± 3.59
23.54 ± 6.6
BLD
BLD
BLD
ALA:LOX
13-HOTrE
180.6 ± 66.42
48.88 ± 24.2
82.62 ± 21.3
BLD
BLD
BLD
ArA:NE
9-HETE
BLD
BLD
BLD
1.59 ± 0.38
2.14 ± 0.63
2.02 ± 0.45
ArA:NE
11-HETE
BLD
BLD
BLD
4.09 ± 1.3
10.6 ± 3.3
7.8 ± 1.6
EPA:NE
15-HEPE
49.35 ± 21.16
19.2 ± 4.6
33.75 ± 6.67
BLD
BLD
BLD
Concentration in μg/g of bladder. h PI: hour postinoculation; PBS: control; ABU: asymptomatic bacteriuric E. coli colonization; UTI-CFT: urinary tract infection with uropathogenic E. coli strain CFT073; ArA: arachidonic acid; LnA: linoleic acid; DHA: docosahexaenoic acid; EPA: eicosapentaenoic acid; ALA: α-linoleic acid; COX: cyclooxygenase; CYP: cytochrome P450 epoxygenase; sEH: soluble epoxide hydrolase; LOX: lipoxygenase; BLD: below limit of detection. Mean and SEM from five mice/group/time point. Chemical names of oxylipids are provided in the Materials and Methods.
