Mold Alkaloid Cytochalasin D Modifies the Morphology and Secretion of fMLP-, LPS-, or PMA-Stimulated Neutrophils upon Adhesion to Fibronectin

Figure 2

Effect of cytochalasin D on the external and internal morphology of LPS-treated neutrophils upon adhesion to fibronectin. SEM (a, b) and TEM (c, d) images of neutrophils that were attached to fibronectin-coated substrata over a period of 20 min in the presence of 20 μM LPS, taken alone (a, c) or together with 10 μM cytochalasin D (b, d) and TEM images of the thin seral sections of the cell that was attached to substrata in the presence of 20M LPS together with 10 μM cytochalasin D (d1–d4). White arrows indicate cytoplasm islands. Black arrows indicate the common exits to the exterior of intracellular vacuoles. Pictures represent typical images observed in two independent experiments.