Research Article

Mold Alkaloid Cytochalasin D Modifies the Morphology and Secretion of fMLP-, LPS-, or PMA-Stimulated Neutrophils upon Adhesion to Fibronectin

Table 1

A list of proteins that were secreted by neutrophils into the extracellular medium during a 20 min incubation with fibronectin-coated substrata under control conditions, in the presence of 1 μM fMLP alone or in the presence of both 1 μM fMLP and 10 μM cytochalasin D (fMLP + Cyt. D). Mass spectrometric analysis data were taken from experiments with fMLP. Analogous proteins that were identified in control experiments or in experiments with fMLP + Cyt. D are marked (+). Mass spectrometric data for proteins that were identified upon fMLP + Cyt. D treatment were taken from these experiments. Proteins were separated by SDS-PAGE and identified by mass spectrometric analysis. Protein was identified by MSMS analysis. Proteins identified in two or three analogous experiments were included in the list.

Entrez IDProtein namePeptides matched/totalCoverage, %MOWSE score
ControlfMLPfMLP + Cyt. D

Granular proteins
GI:33285860MMP-927/10531123
GI:168988718+Albumin22/5634163
GI:186833+Lactoferrin+29/5947160
GI:7245433+NGAL+8/1251130
GI:13399627+Lysozyme+9/285184
GI:7766942+MPO24/8952114
GI:3891975Cathepsin G10/2541105
GI:227250CAP371877
GI:119589996Proteinase 339127
GI:2877Azurocidin1295
GI:254839344HNP 163111
GI:75765819HNP 26295
GI:229858HNP 363111
S100 proteins
GI:82407447+S100A8/A99/2191143
GI:4506773+S100A99/2190136
GI:21614544+S100A89/2359127