Research Article

Mold Alkaloid Cytochalasin D Modifies the Morphology and Secretion of fMLP-, LPS-, or PMA-Stimulated Neutrophils upon Adhesion to Fibronectin

Table 2

A list of proteins that were secreted by neutrophils into the extracellular medium during a 20 min incubation with fibronectin-coated substrata under control conditions, in the presence of 0,1 μM PMA alone or in the presence of both 0,1 μM PMA and 10 μM cytochalasin D (PMA + Cyt. D). Mass spectrometric analysis data were taken from experiments with PMA. Analogous proteins that were identified in experiments with PMA + Cyt. D are marked (+). Proteins were separated by SDS-PAGE and identified by mass spectrometric analysis. Proteins identified in two or three analogous experiments were included in the list.

Entrez IDProtein namePeptides matched/totalCoverage, %MOWSE score
PMAPMA + Cyt. D

Granular proteins
GI:22532481MMP-917/762990
GI:332356380Albumin16/522991
GI:1619857Lactoferrin+34/6750225
GI:4261868NGAL+14/5668109
GI:17942571Lysozyme+5/145373
GI:3891975Cathepsin G13/2337152
S100 proteins
GI:82407447S100A8/A9+15/1169079
GI:4506773S100A9+6/295778
GI:21614544S100A85/145373