Research Article

Mold Alkaloid Cytochalasin D Modifies the Morphology and Secretion of fMLP-, LPS-, or PMA-Stimulated Neutrophils upon Adhesion to Fibronectin

Table 3

A list of proteins that were secreted by neutrophils into the extracellular medium during a 20 min incubation with fibronectin-coated substrata in the presence of 20 μg/mL LPS alone, 10 μM cytochalasin D (Cyt. D) alone, or in the presence of both 20 μg/mL LPS and 10 μM cytochalasin D (LPS + Cyt. D). Mass spectrometric analysis data were taken from experiments with LPS. Analogous proteins that were identified in experiments with LPS + Cyt. D are marked (+). Proteins were separated by SDS-PAGE and identified by mass spectrometric analysis. Protein was identified by MSMS analysis. Proteins identified in two or three analogous experiments were included in the list.

Entrez IDPeptides matched/totalCoverage, %MOWSE score
LPSLPS + Cyt. D

Granular proteins
GI:269849668MMP-922/6930225
GI:113576Albumin21/854099
GI:85700158Lactoferrin+23/7141241
GI:1171700NGAL+10/2860134
GI:48428995Lysozyme C6/373649
GI:129825MPO+16/792198
GI:115725Cathepsin G+10/6438131
GI:30316322Defensin HNP 16/82075
GI:30316323Defensin HNP 36/122068
Cytoskeleton proteins
GI:46397333Beta actin+7/502543
GI:54036678Gamma actin+7/502543
Energy metabolism enzymes
GI:7669492GAPDH1571
S100 proteins
GI:115444S100A98/378194
GI:115442S100A88/1761128