Increased Abundance of Plasmacytoid Dendritic Cells and Interferon-Alpha Induces Plasma Cell Differentiation in Patients of IgA Nephropathy

Figure 7

Transendothelial migration of CD14⁺ cells and CD4⁺ cells was promoted by IFN-α-conditioned HMC supernatant. HUVEC cells were cultured onto inserts of transwells, and the bottom champers were filled with culture supernatant of HMC preconditioned with recombinant IFN-α or vehicles. CD14⁺ cells and CD4⁺ cells were sorted from fresh PBMCs of donors () by flow cytometry and loaded on the upper wells. After migrating for 6–12 h, migrated cells in the bottom champers were collected and counted in high power field.

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