Gαq Regulates the Development of Rheumatoid Arthritis by Modulating Th1 Differentiation

Figure 2

Loss of Gαq enhances the differentiation of Th1 cells. Purified naïve CD4⁺ T cells from WT and Gnaq−/− mice were stimulated with anti-CD3/CD28 (3 μg/mL), in the presence of mouse IL-12 (20 ng/mL), mouse IL-2 (20 ng/mL), and anti-IL-4 (10 μg/mL) for five days. Cells were harvested and analyzed. (a) WT and Gnaq−/− CD4⁺ T cells were stimulated with PMA, ionomycin, and monensin, fixed, permeabilized, and stained with FITC-conjugated anti-IFN-γ, followed by flow cytometry. (b) The percentage of IFN-γ⁺ cells was calculated. (c) IFN-γ secretion was detected by ELISA. Cultured CD4⁺ T cells were harvested, adjusted to same concentration, and stimulated by anti-CD3/CD28 (1 μg/mL) for 24 hours. Supernatants were collected for ELISA assay. All data are presented as mean ± SD; < 0.05, . The result is representative of three independent experiments.

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