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Mediators of Inflammation
Volume 2017, Article ID 4814987, 11 pages
https://doi.org/10.1155/2017/4814987
Research Article

Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis

1Dipartimento di Area Medica (DAME), Università degli Studi di Udine, Udine, Italy
2Clinica Ortopedica, Azienda Sanitaria Universitaria Integrata di Udine, Udine, Italy
3Dipartimento di Ortopedia e Traumatologia, Azienda per l’Assistenza Sanitaria n.3 Alto Friuli, Tolmezzo, Italy
4Istituto di Patologia Clinica, Azienda Sanitaria Universitaria Integrata di Udine, Udine, Italy
5NantBioScience, Inc., Culver City, CA 90232, USA

Correspondence should be addressed to Francesco Curcio; ti.duinu@oicruc.ocsecnarf

Received 1 December 2016; Revised 16 March 2017; Accepted 2 April 2017; Published 28 May 2017

Academic Editor: Amedeo Amedei

Copyright © 2017 Rossana Domenis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to characterize synovial fluid- (SF-) derived exosomes of patients with gonarthrosis comparing two methods of isolation and to investigate their immune regulatory properties. Extracellular vesicles (EVs) have been isolated from inflamed SF by polymer precipitation method and quantified by Exocet kit and by nanoparticle tracking analysis. Vesicles expressed all the specific exosomal markers by immunoblot and FACS. After isolation with Exoquick, a relevant contamination by immune complexes was detected, which required further magnetic bead-based purification to remove. SF-derived exosomes significantly stimulated the release of several inflammatory cytokines and chemokines and metalloproteinases by M1 macrophages but did not influence the expression of CD80 and CD86 costimulatory molecules. In conclusion, we characterized purified exosomes isolated from inflamed SF and demonstrate that purified exosomes are functionally active in their ability to stimulate the release of proinflammatory factors from M1 macrophages. Our data indicate that SF-derived exosomes from gonarthrosis patients play a role in disease progression.