Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

Table 1

List of primer pairs used for quantitative PCR.

Primer	Sequence (5 to 3)	Product length (bp)	Binding site
SREBP-1 forward	CGG AGA AGC TGC CTA TCA AC	379	Exon 5
SREBP-1 reverse	GGT CAG TGT GTC CTC CAC CT		Exon 7
GPAT forward	AGT GAG GAA TGG GGT GAG TG	300	Exons 3 and 4
GPAT reverse	CAG TCA CAT TGG TGG CAA AC		Exon 6
IL-1β forward	CAC ATG GGA TAA CGA GGC TT	147	Exon 5
IL-1β reverse	TTG TTG CTC CAT ATC CTG TCC		Exon 5
IL-6 forward	CTC AGC CCT GAG AAA GGA GA	310	Exons 2 and 3
IL-6 reverse	CAG GGG TGG TTA TTG CAT TCT		Exon 5
IL-8 forward	GTG CAG TTT TGC CAA GGA GT	195	Exon 2
IL-8 reverse	CTC TGC ACC CAG TTT TCC TT		Exon 3
TNF-α forward	CAC TAA GAA TTC AAA CTG GGG C	165	Exon 4
TNF-α reverse	GAG GAA GGC CTA AGG TCC AC		Exon 4
TGF-β forward	GGG ACT ATC CAC CTG CAA GA	420	Exon 1
TGF-β reverse	CAC GTG CTG CTC CAC TTT TA		Exon 2
GAPDH forward	AAA GGA TCC ACT GGC GTC TTC ACC ACC	206	Exon 5
GAPDH reverse	GAA TTC GTC ATG GAT GAC CTT GGC CAG		Exon 7