Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo

Figure 1

(a) Expression of GHS-R1a in the presence and absence of F. nucleatum (Fn; OD₆₆₀: 0.1) in PDL cells at 1 d and 2 d, as analyzed by real-time PCR. Mean ± SEM (). Significant () difference between groups. (b) Expression of GHS-R1a in PDL cells exposed to various concentrations of F. nucleatum (Fn; OD₆₆₀: 0.05, 0.1, and 0.2) at 1 d. Nonstimulated cells served as control. Mean ± SEM (). (c) GHS-R protein synthesis in PDL cells stimulated with F. nucleatum (OD₆₆₀: 0.1) for 1 d, as visualized by immunocytochemistry. Nonstimulated cells served as control. Representative images from one out of three experiments are shown. (d) Expression of GHS-R1a in PDL cells exposed to P. gingivalis (Pg), T. denticola (Td), or A. actinomycetemcomitans Y4 (Aa) (all OD₆₆₀: 0.1) at 1 d, as determined by real-time PCR. Unstimulated cells served as control. Mean ± SEM (). Significantly () different from control.

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