Mitofusin 2 Promotes Apoptosis of CD4⁺ T Cells by Inhibiting Autophagy in Sepsis

Figure 4

Pharmacological inhibition of autophagy enhanced Mfn2 overexpression-induced cell apoptosis. After transfection with a lentiviral vector (LV-Mfn2 or LV-mCherry), Jurkat T cells were divided into three groups and subjected to the following treatments: DMSO (vehicle control), rapamycin (100 nM, an autophagy stimulator), or 3-methyladenine (10 mM, an autophagy inhibitor). (a) The percentage of apoptotic cells based on Annexin-V staining. (b) The expression of the autophagy-related proteins Beclin1, p62, and LC3-II was measured via Western blot analysis. The results are shown as the mean ± SD. Baf A1: bafilomycin A1. RAPA: rapamycin. 3-MA: 3-methyladenine. , significant difference versus the control + DMSO group. ^##, significant difference versus the LV-Mfn2 + DMSO group. ^$, significant difference versus the LV-mCherry + DMSO group. ^$$, significant difference versus the LV-mCherry + DMSO group.

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