Mitofusin 2 Promotes Apoptosis of CD4⁺ T Cells by Inhibiting Autophagy in Sepsis

Figure 5

Overexpression of Mfn2 decreased PMA/ionomycin-, rapamycin-, and starvation-induced autophagy. Jurkat T cells were infected with lentiviral vector and then exposed to autophagy inducers, PMA (50 ng/ml)/ionomycin (1 μM) (a), rapamycin (100 nM) (b), or starvation (low serum) (c), for the indicated intervals, followed by Western blotting to determine the expression levels of the indicated proteins. β-Actin served as a loading control for Beclin1 and p62 protein levels. The results of three independent experiments are shown as the mean ± SD. P/I: PMA/ionomycin. Baf A1 or B: bafilomycin A1. RAPA: rapamycin. ^##, significant difference versus the LV-mCherry group or the LV-mCherry + B group. ^$, significant difference versus the LV-RFP group.

(a)

(b)

(c)