Pretreatment with Fish Oil-Based Lipid Emulsion Modulates Muscle Leukocyte Chemotaxis in Murine Model of Sublethal Lower Limb Ischemia

Table 1

Primer sequences used in the quantitative real-time reverse-transcription polymerase chain reaction assays.

Gene name	5′-3′ primer sequence
KC	F: CTTGAAGGTGTTGCCCTCAG
	R: TGGGGACACCTTTTAGCATC
MCP-1	F: ACTGAAGCCAGCTCTCTCTTCCTC
	R: TTCCTTCTTGGGGTCAGCACAGAC
TNF-α	F: CCCTCACACTCAGATCATCTTCT
	R: GCTACGACGTGGGCTACAG
IL-6	F: GGGACTGATGCTGGTGACAA
	R: ACAGGTCTGTTGGGAGTGGT
Hsp72	F: GCTGGCTAGGAGACAGATATGTGGC
	R: AAAGCCCACGTGCAATACACAAAGT
PPAR-γ	F: GCCCTTTGGTGACTTTATGG
	R: CAGCAGGTTGTCTTGGATGT
GAPDH	F: GAAGGTCGGTGTGAACGGAT
	R: AATCTCCACTTTGCCACTGC

KC, keratinocyte-derived chemokine; MCP-1, monocyte chemotactic protein-1; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; Hsp72, heat shock protein 72; PPAR-γ, peroxisome proliferator-activated receptor-γ; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.