Nuclear Translocation of SGPP-1 and Decrease of SGPL-1 Activity Contribute to Sphingolipid Rheostat Regulation of Inflammatory Dendritic Cells

Figure 1

(a, b) Quantitative real-time PCR for sgpl-1 and sgpp-1 mRNA in naive and LPS-stimulated dendritic cells over time (). (c) Western blot with anti-SGPL-1 antibody and anti-β-actin antibody in naive and LPS-stimulated DCs after 48 h. (d) Quantification of hexadecenal for SGPL-1 activity in naive and LPS-stimulated dendritic cells with QTOF-MS (). (e) Western blot for anti-SGPP-1 antibody and anti-β-actin antibody in naive and LPS-stimulated DCs after 48 h (). (f) Quantification of Western blot bands of (e) (statistical test: one sample t-test). Data represent mean ± SD; and .

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