Nuclear Translocation of SGPP-1 and Decrease of SGPL-1 Activity Contribute to Sphingolipid Rheostat Regulation of Inflammatory Dendritic Cells

Figure 3

(a–f) Representative pictures of confocal laser microscopy of (a) untreated mature GM-CSF-differentiated murine bone marrow-derived dendritic cells stained with murine anti-SGPP-1 antibody (green) and DAPI (blue) (), (b) untreated mature human monocyte-derived dendritic cells stained with human anti-SGPP-1 antibody (green) and DAPI (blue) (), (c) untreated mature murine GM-CSF-differentiated dendritic cells (basal), after 48 h of starvation (naive) and 48 h of LPS stimulation (inflammatory) stained with DAPI (blue) and anti-SGPP-1 antibody (green) as indicated (), and (d–f) mature GM-CSF-differentiated murine dendritic cells stained with ER tracker (pseudocolor red) and anti-SGPP-1 antibody (green) (d) under basal conditions, (e) after 24 h of LPS stimulation, and (f) after 48 h of LPS stimulation ().