Kaempferide Protects against Myocardial Ischemia/Reperfusion Injury through Activation of the PI3K/Akt/GSK-3β Pathway

Figure 3

Kae treatment increased the levels of phosphorylated Akt and phosphorylated GSK-3β in the myocardium. PI3K inhibition (LY294002) attenuates Kae-induced increases in phospho-Akt and phospho-GSK-3β levels. (a) Representative Western blots for Akt, p-Akt, GSK-3β, and P-GSK-3β. (b) Grey value analysis demonstrates that Kae treatment increases the ratio of p-Akt/Akt. (c) Grey value analysis demonstrates that Kae treatment increases the ratio of p-GSK-3β/GSK-3β. ^## versus sham; versus I/R; ⁺⁺ versus H-Kae + I/R.

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