Research Article
Kaempferide Protects against Myocardial Ischemia/Reperfusion Injury through Activation of the PI3K/Akt/GSK-3β Pathway
Figure 5
Kae preconditioning significantly inhibited cardiomyocyte apoptosis, decreased the levels of cleaved caspase-3, and increased caspase-3 levels. Moreover, these effects of Kae treatment were reversed by cotreatment with TDZD-8. (a) Suppression of cardiomyocyte apoptosis by Kae treatment (×100). Brown staining of the nucleus is indicative of apoptosis. (b) Percentage of apoptotic cells. (c) Cleaved caspase-3 and caspase-3 protein levels in rat hearts were analyzed by Western blot. Expression of cleaved caspase-3 (d) and caspase-3 (e) in tissue of rat hearts measured by Western blot analysis ( in each group). ## versus sham; versus I/R; + and ++ versus H-Kae + I/R.
(a) |
(b) |
(c) |
(d) |
(e) |