Research Article
Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
Figure 1
Construction of plasmid pORF-mIL-12-ORT by standard cloning methods and operator-repressor titration (ORT) technology. AmpR: ampicillin resistance gene; CM: chloramphenicol resistance gene; Kan: kanamycin resistance gene.