Research Article

Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma

Table 1

The experimental groups and the number of mice sacrificed at each time point.

GroupTime intervals
1 day4 days8 days11 days14 days85 days

ā€‰ā€‰Number of mice
(1) Negative control groupSkin2
(2) Positive control groupSkin + LPS222020
(3) Control group: H2OTumors + endotoxin-free water application665000
(4) Control group: H2O + EPTumors + endotoxin-free
water application + EP pulses
666000
(5) Control group: pControl + EPTumors + control plasmid + EP pulses664500
(6) Therapeutic group: pORF-mIL-
12-ORT + EP
Tumors + therapeutic
plasmid + EP pulses
676078